In this study, the viability of the kenaf fibre use, alone or combined with cereal straw, vine shoots and olive mill dried waste, in the elaboration of specific substrates for the cultivation of Pleurotus ostreatus (Jacq. ex Fr.) Kummer, second mushroom in importance cultivated in Spain, is described. Furthermore, three different methods of preparation of the substrate have been considered in order to obtain selectivity for the growth and later fruiting of Pleurotus sporophore. As for the production parameters, the best results have been provided by the substrates that combined kenaf with straw and with vine shoots, being unfavourable the substrates based in just kenaf or combined with olive mill dried waste. As for the treatment applied to the materials, the immersion in water alone and subsequent pasteurization and thermophilic conditioning, together with the semi-anaerobic fermentation, has been favoured in front of the immersion in water with fungicide and later pasteurization.

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